It comes after new figures showed how much money is invested in different projects across sectors including, young people, employment and agriculture.
Since 2017, around £93 million has been granted for more than 3,000 farmers in the North East.
And around £80 million has been given for research projects as well as £5 million for growth and jobs.
A number of organisations benefit from funding as well as universities, colleges and schools.
Now Banffshire and Buchan Coast MSP Stewart Stevenson has warned projects may face a gap in investment post-Brexit.
The EU invests around £5 billion a year in the UK and the European single market is around eight times bigger than the UK market alone.
Commenting, Banffshire and Buchan Coast MSP Stewart Stevenson said:
“As these figures show, the North East and its surrounding areas have benefitted hugely from this cash.
“This funding has made a huge contribution to infrastructure, culture, and opportunities in Scotland. This is just the latest analysis which confirms that UK Tory Government's mishandling of Brexit is going to inflict major harm on our economy.
“Far from delivering extra cash for the NHS, as was infamously promised by the Leave campaign bus, Scotland faces losing out on billions of pounds of funding with no clarity from the UK government over how this will be replaced.”
“This funding has made a huge contribution to infrastructure, culture, and opportunities in Scotland. This is just the latest analysis which confirms that UK Tory Government's mishandling of Brexit is going to inflict major harm on our economy.
“Far from delivering extra cash for the NHS, as was infamously promised by the Leave campaign bus, Scotland faces losing out on billions of pounds of funding with no clarity from the UK government over how this will be replaced.”